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KMID : 0364820130490040328
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Korean Journal of Microbiology
2013 Volume.49 No. 4 p.328 ~ p.335
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Phylogenetic Analysis of Bacterial Populations in a Tomato Rhizosphere Soil Treated with Chicken Feather Protein Hydrolysate
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Kim Se-Jong
Han Song-Ih
Whang Kyung-Sook
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Abstract
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As a result of conducting a cultural experiment of tomato using chicken feather protein hydrolysate (CPH) which
was mass produced by keratin protein degrading bacterium Chryseobacterium sp. FBF-7 (KACC 91463P), we found
that the stem and the root of tomato showed significant improvement in growth. For the purpose of phylogenic
interpretation, a comparison was drawn between the effect of CPH, a treated CPH and untreated, on the changes of
bacterial populations by 454 pyrosequencing based on 16S rRNA gene sequences. Tomato rhizosphere soil untreated
with CPH (NCPH) showed 6.54 Shannon index from 3,281 sequence reads, and the rhizosphere soil treated with
CPH (TCPH) showed 6.33 Shannon index from 2,167 sequence reads, displaying that it does not affect the diversity.
Bacterial populations were composed of 19 phyla in the rhizosphere soil, and the phylum Proteobacteria occupied
40% of total bacterial populations. Bradyrhizobium, Agromonas, Nitrobacter, and Afipia (BANA group) which
belong to Bradyrhizobiaceae were abundant and commonly detected in both the treated and untreated soils,
suggesting the dominance of bacterial group in rhizosphere soil. The results obtained showed that CPH treatment
does not affect the indigenous bacterial populations present in the rhizosphere soil.
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KEYWORD
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Bradyrhizobiaceae, chicken feather protein hydrolysate, pyrosequencing, tomato rhizosphere soil
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